Another run with 12 samples was performed on the 32-well block during which a temperature of 99°C was maintained on the 64-well block. Results and discussion (1) PCR optimization via gradient function ...
Producing high yields of good-quality PCR products requires a complex combination of optimal chemistry, primer design, template quality, and cycling parameters. The details of these reaction ...
MRD detection by PCR analysis of rearranged Ig/TCR genes is however a complex process, involving many steps (Figure 1). Identification of pitfalls in this process is of importance in order to ensure ...
Delivering the correct volume of master mix has a significant impact on the quality of qPCR test results. When running automated PCR assays, laboratories have not had an efficient means of tuning in ...
The 2X PCR Master Mix is a made-to-order custom master mix. Once your order is received, your custom master mix will be delivered in 10 business days. The 2X formulation contains customer defined ...
Common problems that arise during PCR assays include weak target-binding, competing target secondary structure, primer-dimer formation, and mishybridization of PCR probes and primers. These issues, ...
The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...
SuperScript IV UniPrime One-Step RT-PCR System is designed to perform both cDNA synthesis and subsequent PCR amplification in a single tube. This master mix formulation combines the high processivity ...
PCR is a technique used to amplify target DNA in a sample. It’s a well-known method that has undergone numerous modifications to enhance its capabilities. This year, it’s turning 40 years old. PCR has ...
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