May 8, 2009 · Hi, I have a very basic question. I am starting to do cell cultures and I have come across different ratios. What does 1:3 ratio mean? How to passage you cells in 1:3 ratio or say …

Does higher volumes mean better cell yield ? I need to retain as many cells in the pellet as possible and lose few as possible during the wash and centrifugation. I think the volume you …

I was trying to calculate it out, but not sure how much final volume you are making up, so could not figure out how you got to your resuspension value of 285ul. In what I described, I am …

May 6, 2012 · What does percentage mean when dealing with stacking and running gel for SDS page? In a lot of ways, I'm trying to figure out why particular amounts of chemicals are used. …

Jun 8, 2007 · SmoochiePie, wt/vol is just a way of expressing weight by volume concentration. Percent concentration can be expressed in other ways too. e.g. %w/w. For a 10%wt/vol …

The volume of the primers used must be suitably low, but not too low, to be pipetted accurately. For example: Stock solution of each primer is usually 100µM (100pmol/µl). You make a …

Washing is washing.. its cleaning the pellet by adding a product (dont know what you use?). You will not "move" or break the pellet , you just add a substance to clean the pellet (remove …

1:5 means that you divide the cell volume you have by 5 and add one part to a new plate/well/flask... and is completely independent of the volume you are adding it to.

I do not understand the 10ug of 200ng/ul DNA? you mean that they started with 1 g in the PCR and because of you used the PCR you ended up with 10 g in the end? And what about the …

UPDATE Tue 12th June 2012 I have used MilliQ water to pump off this horrendous brown/black stuff - two column volume, and thank heavens it comes off. Next I pumped 2 column volumes …